Molecular characterization of a population-based series of endometrial stromal sarcomas in Kuwait
Affiliations
Affiliations
- Department of Pathology, Faculty of Medicine, Health Sciences Center, Kuwait University, Safat 13110, Kuwait. Electronic address: rolapath@hotmail.com.
- Department of Pathology, Maternity Hospital, Safat 13001, Kuwait.
- Department of Pathology, Faculty of Medicine, Health Sciences Center, Kuwait University, Safat 13110, Kuwait.
- Department of Obstetrics and Gynecology, Faculty of Medicine, Health Sciences Center, Kuwait University, Safat 13110, Kuwait.
- Centre for Translational and Applied Genomics, British Columbia Cancer Agency, Vancouver, BC V5Z 4E6, Canada.
- Department of Laboratory Medicine and Pathology, University of Alberta and Royal Alexandra Hospital, Edmonton, AB T5H 3V9, Canada.
Abstract
Endometrial stromal sarcomas (ESSs) frequently harbor genetic fusions, including JAZF1-SUZ12 and equivalent fusions in low-grade ESS (LGESS) and YWHAE-NUTM2 in high-grade ESS (HGESS). This study aims to classify a population-based series of ESSs in Kuwait based on the 2014 World Health Organization classification system and to assess the diagnostic use of interferon-induced transmembrane protein 1 (IFITM1) immunomarker for ESSs. Twenty ESSs including 19 LGESSs and 1 HGESS treated during the period between 2002 and 2013 were identified, and the cases were reviewed and characterized using fluorescence in situ hybridization and immunohistochemical studies. Thirteen (81.3%) of 16 LGESSs with interpretable results showed JAZF1 and/or PHF1 genetic rearrangements by fluorescence in situ hybridization, and the only HGESS in the series showed YWHAE genetic rearrangement. All LGESSs with interpretable results showed positive immunostaining for CD10 compared with 11 (61%) of 18 that showed positive immunostaining for IFITM1; 4 of 7 IFITM1-negative LGESSs showed JAZF1 and/or PHF1 rearrangements. A series of uterine leiomyomas, leiomyosarcomas, adenosarcomas, and carcinosarcomas were included for comparison, and positive IFITM1 staining was found in 1 of 10 leiomyomas, 3 of 13 leiomyosarcomas, 3 of 4 adenosarcomas, and 3 of 8 carcinosarcomas, compared to 0 of 10 leiomyomas, 9 of 13 leiomyosarcomas, 3 of 4 adenosarcomas, and 5 of 8 carcinosarcomas that were positive for CD10. Our results demonstrated characteristic genetic rearrangements in a high percentage of LGESSs in this Middle Eastern population, and IFITM1 antibody appears to be less sensitive than CD10 for LGESS.
Keywords: Endometrial stromal sarcoma; FISH; Genetic fusion; IFITM1; JAZF1; YWHAE.
Similar articles
Isphording A, Ali RH, Irving J, Goytain A, Nelnyk N, Hoang LN, Gilks CB, Huntsman DG, Nielsen TO, Nucci MR, Lee CH.Hum Pathol. 2013 May;44(5):837-43. doi: 10.1016/j.humpath.2012.08.007. Epub 2012 Nov 15.PMID: 23159154
Lee CH, Ali RH, Rouzbahman M, Marino-Enriquez A, Zhu M, Guo X, Brunner AL, Chiang S, Leung S, Nelnyk N, Huntsman DG, Blake Gilks C, Nielsen TO, Dal Cin P, van de Rijn M, Oliva E, Fletcher JA, Nucci MR.Am J Surg Pathol. 2012 Oct;36(10):1562-70. doi: 10.1097/PAS.0b013e31825fa931.PMID: 22982899 Free PMC article.
Identification of a novel, recurrent MBTD1-CXorf67 fusion in low-grade endometrial stromal sarcoma.
Dewaele B, Przybyl J, Quattrone A, Finalet Ferreiro J, Vanspauwen V, Geerdens E, Gianfelici V, Kalender Z, Wozniak A, Moerman P, Sciot R, Croce S, Amant F, Vandenberghe P, Cools J, Debiec-Rychter M.Int J Cancer. 2014 Mar 1;134(5):1112-22. doi: 10.1002/ijc.28440. Epub 2013 Sep 4.PMID: 23959973
Endometrial stromal sarcoma--the new genetic paradigm.
Lee CH, Nucci MR.Histopathology. 2015 Jul;67(1):1-19. doi: 10.1111/his.12594. Epub 2015 Jan 22.PMID: 25355621 Review.
Endometrial stromal tumours revisited: an update based on the 2014 WHO classification.
Ali RH, Rouzbahman M.J Clin Pathol. 2015 May;68(5):325-32. doi: 10.1136/jclinpath-2014-202829. Epub 2015 Jan 16.PMID: 25595274 Review.
Cited by
JAZF1/SUZ12 gene fusion in endometrial stromal sarcomas.
Hrzenjak A.Orphanet J Rare Dis. 2016 Feb 16;11:15. doi: 10.1186/s13023-016-0400-8.PMID: 26879382 Free PMC article. Review.