Early initiation of insulin attenuates histological and functional changes in the liver of streptozotocin-induced diabetic rats using 99mTc-sulfur colloid functional imaging
Affiliations
Affiliations
- Department of Nuclear Medicine, Faculty of Medicine, Kuwait University, Kuwait city, Kuwait.
- Department of Pathology, Faculty of Medicine, Kuwait University, Kuwait city, Kuwait.
- Department of Biological Sciences, College of Science, King Faisal University, Al Ahsa, Saudi Arabia.
- Department of Anatomy, Faculty of Medicine, Kuwait University, Kuwait city, Kuwait.
- Royal College of Surgeons in Ireland, Dublin, Ireland.
Abstract
This study aimed to investigate the effect of insulin on the reticuloendothelial system (RES) in the liver and spleen in diabetic rats. Sprague Dawley rats were divided into control, diabetic rats (DM) and diabetic rats treated with insulin (IDM) for 2 weeks. Rats were imaged with technetium-99m-sulfur colloid (99mTc-SC) tracer to determine regional distributions of the tracer for all groups by drawing regions of interest and then obtained the ratios as the cumulative counts of heart, liver, and spleen to the whole body (WB). Liver tissue from sacrificed rats from each group was examined by light and electron microscopy. 99mTc-SC uptake ratios showed a lower liver to WB uptake ratio in the DM rats compared to both controls and IDM rats. Electron microscopy showed severe vacuolization of the hepatocytes of DM rats. The IDM rats show complete resolution of the vacuolization. The early administration of insulin for 2 weeks to diabetic rats could significantly resolve the phagocytic RES function and histological changes in the liver.
Keywords: Diabetes; Sprague Dawley rat; histopathology; insulin; phagocytic cells.
Similar articles
Al-Saeedi F, Loutfi I.Med Princ Pract. 2011;20(2):129-32. doi: 10.1159/000321216. Epub 2011 Jan 20.PMID: 21252566
Assessment of functional liver reserve: old and new in 99mTc-sulfur colloid scintigraphy.
Matesan MM, Bowen SR, Chapman TR, Miyaoka RS, Velez JW, Wanner MF, Nyflot MJ, Apisarnthanarax S, Vesselle HJ.Nucl Med Commun. 2017 Jul;38(7):577-586. doi: 10.1097/MNM.0000000000000695.PMID: 28591006
Al-Saeedi FJ, Cheng B.Clin Physiol Funct Imaging. 2013 Jul;33(4):293-301. doi: 10.1111/cpf.12027. Epub 2013 Jan 24.PMID: 23692619
Mechanism of hepatic extraction of gelatinized 99m technetium sulfur colloid.
George EA, Hendershott LR, Klos DJ, Donati RM.Eur J Nucl Med. 1980 Jun;5(3):241-5. doi: 10.1007/BF00271906.PMID: 7389724
Rydén S, Strand SE, Palmer J, Stenram U, Hafström L, Persson B.Eur J Nucl Med. 1982;7(1):16-21. doi: 10.1007/BF00275239.PMID: 7060599