Emergence of New Sequence Type OXA-48 Carbapenemase-Producing Enterobacteriaceae in Kuwait
Affiliations
Affiliations
- Department of Microbiology, Faculty of Medicine, Kuwait University, Jabriya, Kuwait.
- Microbiology Unit, Mubarak Al-Kabir Hospital, Jabriya, Kuwait.
- Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
- INSERM U914, South-Paris Medical School, K.-Bicêtre, Paris, France.
Abstract
The aim of this study was to investigate the infections due to OXA-48 carbapenemase-producing bacteria in tertiary hospitals in Kuwait (September 2011 to April 2013) and to determine the sequence types (STs) of the corresponding isolates. Eleven OXA-48 carbapenemase-producing Enterobacteriaceae isolates were recovered from patients treated in nine different hospitals in Kuwait. Susceptibility testing to eighteen antibiotics was done using the E-test. PCR assays were performed for the detection of genes encoding extended-spectrum β-lactamases (ESBLs) (blaCTX-M, blaSHV, and blaTEM) and carbapenemases (blaOXA-48, blaVIM, blaNDM, blaIMP, blaGIM, and blaKPC). STs were determined by Multilocus Sequence Typing. Seven Klebsiella pneumoniae, two Escherichia coli, one Enterobacter cloacae, and one Morganella morganii harbored the blaOXA-48 gene. The K. pneumoniae and E. coli belonged to seven and two different STs, respectively, which were not related to those reported from this region. The majority of the isolates carried either blaCTX-M or blaSHV genes and showed a multidrug-resistant phenotype, including resistance to tigecycline. Multidrug-resistant Enterobacteriaceae harboring the blaOXA-48 gene are emerging in Kuwait with different STs compared to those identified in other countries of the region. Detection of OXA-48-producing Enterobacteriaceae in Kuwait is important to prevent its rapid spread.
Similar articles
Alsharapy SA, Gharout-Sait A, Muggeo A, Guillard T, Cholley P, Brasme L, Bertrand X, Moghram GS, Touati A, De Champs C.Microb Drug Resist. 2020 Mar;26(3):211-217. doi: 10.1089/mdr.2018.0443. Epub 2019 Aug 9.PMID: 31397629
Emergence of Enterobacteriaceae co-producing CTX-M-15, ArmA and PMQR in Poland.
Piekarska K, Zacharczuk K, Wołkowicz T, Wolaniuk N, Rzeczkowska M, Gierczyński R.Adv Clin Exp Med. 2019 Feb;28(2):249-254. doi: 10.17219/acem/94165.PMID: 30499638
Mathlouthi N, Al-Bayssari C, El Salabi A, Bakour S, Ben Gwierif S, Zorgani AA, Jridi Y, Ben Slama K, Rolain JM, Chouchani C.J Infect Dev Ctries. 2016 Aug 2;10(7):718-27. doi: 10.3855/jidc.7426.PMID: 27482803
Plasmid evolution in carbapenemase-producing Enterobacteriaceae: a review.
Kopotsa K, Osei Sekyere J, Mbelle NM.Ann N Y Acad Sci. 2019 Dec;1457(1):61-91. doi: 10.1111/nyas.14223. Epub 2019 Aug 30.PMID: 31469443 Review.
Recent advances in the laboratory detection of carbapenemase-producing Enterobacteriaceae.
Matsumura Y, Pitout JD.Expert Rev Mol Diagn. 2016 Jul;16(7):783-94. doi: 10.1586/14737159.2016.1172964. Epub 2016 Apr 18.PMID: 27042955 Review.
Cited by
Alemayehu E, Fiseha T, Gedefie A, Alemayehu Tesfaye N, Ebrahim H, Ebrahim E, Fiseha M, Bisetegn H, Mohammed O, Tilahun M, Gebretsadik D, Debash H, Gobezie MY.BMC Infect Dis. 2023 May 3;23(1):277. doi: 10.1186/s12879-023-08237-5.PMID: 37138285 Free PMC article.
Al Dabbagh M, Alghounaim M, Almaghrabi RH, Dbaibo G, Ghatasheh G, Ibrahim HM, Aziz MA, Hassanien A, Mohamed N.Infect Dis Ther. 2023 May;12(5):1217-1235. doi: 10.1007/s40121-023-00799-w. Epub 2023 Apr 18.PMID: 37071349 Free PMC article. Review.
Villanueva-Cotrina F, Condori DM, Gomez TO, Yactayo KM, Barron-Pastor H.Infect Chemother. 2022 Dec;54(4):765-773. doi: 10.3947/ic.2022.0135.PMID: 36596684 Free PMC article.
Al-Sweih N, Jamal W, Mokaddas E, Habashy N, Kurdi A, Mohamed N.JAC Antimicrob Resist. 2021 Dec 6;3(4):dlab159. doi: 10.1093/jacamr/dlab159. eCollection 2021 Dec.PMID: 36479234 Free PMC article.
Emerging Status of Multidrug-Resistant Bacteria and Fungi in the Arabian Peninsula.
Borgio JF, Rasdan AS, Sonbol B, Alhamid G, Almandil NB, AbdulAzeez S.Biology (Basel). 2021 Nov 6;10(11):1144. doi: 10.3390/biology10111144.PMID: 34827138 Free PMC article. Review.