Assessment of salivary alpha amylase and mucin-4 before and after non-surgical treatment of peri-implant mucositis

Hajer A Aldulaijan 1, Abeer S Al-Zawawi 2, Marwa Y Shaheen 2, Dena Ali 3, Darshan Devang Divakar 4 5, Amani M Basudan 2

Affiliations

doi: 10.1186/s40729-022-00429-z

Abstract

Background: The present study was based on the null hypothesis that there is no difference in clinicoradiographic parameters and whole salivary alpha amylase (AA) and mucin-4 levels before and after non-surgical mechanical debridement (NSMD) of patients with peri-implant mucositis (PM). The aim was to assess whole salivary AA and mucin-4 levels before and after treatment of PM.

Methods: Patients with PM (Group-1) and individuals without peri-implant diseases (Group-2) were included. Demographic data was collected and peri-implant modified plaque and bleeding indices (mPI and mBI, respectively), probing depth (PD) and crestal bone loss were measured at baseline. Levels of AA and mucin-4 were assessed in unstimulated whole saliva samples. All patients underwent full-mouth non-surgical periodontal therapy (NSPT) and NSMD; and clinical parameters and salivary biomarkers were re-assessed after 3 months. Level of significance was set at P < 0.01.

Results: Twenty-six and 32 individuals were included in groups 1 and 2, respectively. None of the participants had periodontitis. At baseline clinical periodontal parameters (PI [P < 0.001], GI [P < 0.001], clinical AL [P < 0.001] and PD [P < 0.001]) were significantly high in Group-1 than Group-2. At 3-month follow-up, there was a statistically significant reduction in clinical periodontal and peri-implant parameters (PI [P < 0.01], GI [P < 0.01], and PD [P < 0.01]) in Group-1 compared with their baseline values. At baseline, salivary AA levels were significantly high in Group-1 than Group-2 (P < 0.01). At 3-month follow-up, there was no significant difference in whole salivary AA levels among patients in groups 1 and 2.

Conclusions: The AA and mucin-4 levels are potential biomarkers for evaluation of peri-implant diseases including PM. Mechanical instrumentation continues to be the most predictable treatment option for the management of peri-implant diseases.

Keywords: Alpha amylase; Dental implant; Mechanical debridement; Mucin-4; Peri-implant mucositis; Whole saliva.

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1 Logistic regression analysis for the correlation between whole salivary alpha amylase and mucin-4 levels and peri-implant probing depth in groups 1 and 2 at baseline

Fig. 1 Logistic regression analysis for the correlation between whole salivary alpha amylase and mucin-4 levels and peri-implant probing depth in groups 1 and 2 at baseline

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