A New Technique to Induce Experimental Myointimal Hyperplasia

Affiliations

01 January 2018

-

doi: 10.1159/000492575


Abstract

Background: Arterial myointimal hyperplasia (MIH) has a significant impact on the long-term outcomes of vascular procedures such as bypass surgery and angioplasty. In this study, we describe a new and innovative technique to induce MIH using a dental flossing cachet in Wistar rats.

Methods: The intimal damage in the common carotid artery was induced by inserting the tip of the dental flossing cachet through the external carotid artery into the common carotid artery and turning it on for 3 rounds of 20 s each (n = 10). After 2 weeks, the rats were anesthetized and the common carotid arteries of the experimental side and the contralateral side (control) were harvested and preserved for histopathological studies.

Results: The experimental carotid arteries showed significant intimal proliferation and thickening compared to the controls. The intima/media ratio of the experimental and normal (control) common carotid arteries were 1.274 ± 0.162 and 0.089 ± 0.023 (mean ± SEM), respectively (p < 0.001).

Conclusion: This technique is simple, inexpensive, and highly reproducible and it induces sufficient MIH to study this phenomenon in animal models.

Keywords: Angioplasty; Carotid stenosis; Intimal injury; Myointimal hyperplasia; Neointima formation.


Similar articles

Pharmacologic inhibition of nitric oxide synthases and cyclooxygenases enhances intimal hyperplasia in balloon-injured rat carotid arteries.

Fischer JW, Hawkins S, Clowes AW.J Vasc Surg. 2004 Jul;40(1):115-22. doi: 10.1016/j.jvs.2004.03.037.PMID: 15218471 Free PMC article.

Inhibitory effect of adenosine on intimal hyperplasia and proliferation of smooth muscle cells in a carotid arterial anastomosis animal model.

Albayrak G, Silistreli E, Ergur B, Kalkan S, Karabay O, Erdal AC, Acikel U.Vascular. 2015 Apr;23(2):124-31. doi: 10.1177/1708538114533962. Epub 2014 May 6.PMID: 24803551

Effect of the P2Y12 antagonist ticagrelor on neointimal hyperplasia in a rabbit carotid anastomosis model†.

Sürer S, Toktas F, Ay D, Eris C, Yavuz S, Turk T, Vural AH, Goncu MT, Gul NY, Yalcınkaya U.Interact Cardiovasc Thorac Surg. 2014 Aug;19(2):198-204. doi: 10.1093/icvts/ivu087. Epub 2014 May 29.PMID: 24876217

Hemodynamic Parameters and Early Intimal Thickening in Branching Blood Vessels.

Kleinstreuer C, Hyun S, Buchanan JR Jr, Longest PW, Archie JP Jr, Truskey GA.Crit Rev Biomed Eng. 2017;45(1-6):319-382. doi: 10.1615/CritRevBiomedEng.v45.i1-6.140.PMID: 29953383 Review.

Idiopathic myointimal hyperplasia of mesenteric veins.

Platz J, Hyman N.Gastroenterol Hepatol (N Y). 2012 Oct;8(10):700-2.PMID: 24683382 Free PMC article.


KMEL References