Prevalence of target anaerobes associated with chronic periodontitis

Ameerah M Alazemi 1, W Jamal 1, A Al Khabbaz 2, V O Rotimi 1

Affiliations

doi: 10.1099/acmi.0.000177

Abstract

Introduction: Periodontal diseases are a group of chronic infections that destroy tissues surrounding and supporting the teeth. Data on the anaerobes associated with periodontal infections in Kuwait is lacking.

Aim: To investigate the target anaerobes associated with chronic periodontitis (CP) in patients admitted to Dental Clinics in Kuwait University Health Sciences Center, Kuwait.

Methodology: Patients with CP (severe and moderate) were recruited into this study during a period of 15 months. Samples were collected directly from inside the gingival pockets and subjected to semi-quantitative PCR assays.

Results: A total of 30 patients, stratified into moderate and severe CP and 31 healthy individuals, used as controls, were studied. Nine (30 %) of the 30 patients were in the 50-59-year age group. The detection rate of Aggregatibacter actinomycetemcomitans between the patients (9 : 30 %) versus the controls (5 : 16.1 %) was non-significant (P >0.05). Fusobacterium spp., were detected in all patients versus 29 (93.1 %) controls, (P >0.05). However, four target anaerobes were significantly associated with CP patients; Porphyromonas gingivalis was detected in ten (33.3 %) patients versus two (6.4 %) controls (P <0.0001); Tannerella forsythia 25 (83.3 %) versus 16 (51.6 %) controls (P <0.0001); Parvimonas micra 27 (90 %) versus 16 (51.6 %) controls (P <0.0001) and Treponema denticola, 18 (60 %) versus nine (29 %) controls (P <0.0001), respectively. Prevotella spp. were detected in 27 (90 %) patients and 30 (96.7 %) controls (P>0.5). There was no significant difference in the burden of Prevotella spp. between patients and controls determined by semi-quantitative PCR assays.

Conclusion: Some (4/7) of the target anaerobes were significantly associated with CP in our study. P. gingivalis was the most strongly associated anaerobe with CP, although not the keystone bacteria, while Prevotella spp. was similar to the healthy controls.

Keywords: Anaerobes; etiology; periodontal infections; Kuwait.

Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Fig. 1. Comparison of DNA concentration of Prevotella spp. in healthy individuals, moderate periodontitis and severe periodontitis.

Fig. 1. Comparison of DNA concentration of Prevotella spp. in healthy individuals, moderate periodontitis and severe periodontitis.

Similar articles

Periodontopathogens and human β-defensin-2 expression in gingival crevicular fluid from patients with periodontal disease in Guangxi, China.

Yong X, Chen Y, Tao R, Zeng Q, Liu Z, Jiang L, Ye L, Lin X.J Periodontal Res. 2015 Jun;50(3):403-10. doi: 10.1111/jre.12220. Epub 2014 Jul 28.PMID: 25065393

[Study on the detection of P. gingivalis, A. actinomycetemcomitans and T. denticola and the correlation between coinfections of the microbes and levels of chronic periodontitis lesion].

Zhan DF, Liu ZW, Xia XP, Hu JC, Chen LL, Yan J.Zhonghua Liu Xing Bing Xue Za Zhi. 2005 Feb;26(2):120-3.PMID: 15921614 Chinese.

A three-year study on periodontal microorganisms of short locking-taper implants and adjacent teeth in patients with history of periodontitis.

Yan X, Lu H, Zhang L, Zhu B, Piao M, Huang B, Zhang H, Meng H.J Dent. 2020 Apr;95:103299. doi: 10.1016/j.jdent.2020.103299. Epub 2020 Feb 15.PMID: 32070755

Prevalence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythia in Japanese patients with generalized chronic and aggressive periodontitis.

Tomita S, Komiya-Ito A, Imamura K, Kita D, Ota K, Takayama S, Makino-Oi A, Kinumatsu T, Ota M, Saito A.Microb Pathog. 2013 Aug-Sep;61-62:11-5. doi: 10.1016/j.micpath.2013.04.006. Epub 2013 Apr 20.PMID: 23608307

Detection of eight periodontal microorganisms and distribution of Porphyromonas gingivalis fimA genotypes in Chinese patients with aggressive periodontitis.

Feng X, Zhang L, Xu L, Meng H, Lu R, Chen Z, Shi D, Wang X.J Periodontol. 2014 Jan;85(1):150-9. doi: 10.1902/jop.2013.120677. Epub 2013 May 7.PMID: 23646850

Cited by

Prevalence of Periodontal Pathogens in Slovak Patients with Periodontitis and Their Possible Aspect of Transmission from Companion Animals to Humans.

Sondorová M, Kučera J, Kačírová J, Krchová Nagyová Z, Šurín Hudáková N, Lipták T, Maďar M.Biology (Basel). 2022 Oct 19;11(10):1529. doi: 10.3390/biology11101529.PMID: 36290432 Free PMC article.

KMEL References

https://pubmed.ncbi.nlm.nih.gov/

References

  1.  
    1. Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol. 2010;8:481–490. doi: 10.1038/nrmicro2337. - DOI - PubMed
  2.  
    1. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL. Microbial complexes in subgingival plaque. J Clin Periodontol. 1998;25:134–144. doi: 10.1111/j.1600-051X.1998.tb02419.x. - DOI - PubMed
  3.  
    1. Haffajee AD, Bogren A, Hasturk H, Feres M, Lopez NJ, et al. Subgingival microbiota of chronic periodontitis subjects from different geographic locations. J Clin Periodontol. 2004;31:996–1002. doi: 10.1111/j.1600-051X.2004.00597.x. - DOI - PubMed
  4.  
    1. Al-Hebshi NN, Shuga-Aldin HM, Al-Sharabi AK, Ghandour I. Subgingival periodontal pathogens associated with chronic periodontitis in Yemenis. BMC Oral Health. 2014;14:13. doi: 10.1186/1472-6831-14-13. - DOI - PMC - PubMed
  5.  
    1. American Academy of Periodontology Task force report on the update to the 1999 classification of periodontal diseases and conditions. J Periodontol. 2015;86:835–838. doi: 10.1902/jop.2015.157001. - DOI - PubMed
  6.  
    1. Rafiei M, Kiani F, Sayehmiri K, Sayehmiri F, Tavirani M, et al. Prevalence of anaerobic bacteria (P.gingivalis) as major microbial agent in the incidence periodontal diseases by meta-analysis. J Dent. 2018;19:232–242. doi: 10.1128/JCM.43.8.3944-3955.2005. - DOI - PMC - PubMed
  7.  
    1. Eke PI, Dye BA, Wei L, Thornton-Evans GO, Costa FO, et al. Prevalence of periodontitis in adults in the United States: 2009 and 2010. J Dent Res. 2012;91:914–920. doi: 10.1590/S1806-83242010000200015. - DOI - PubMed
  8.  
    1. Faghri J, Moghim S, Abed AM, Rezaei F, Chalabi M, et al. Prevalence of Porphyromonas gingivalis and Bacteroides forsythus in chronic periodontitis by multiplex PCR. Pak J Biol Sci. 2007;10:4123–4127. doi: 10.3923/pjbs.2007.4123.4127. - DOI - PubMed
  9.  
    1. Kumar PS, Griffen AL, Moeschberger ML, Leys EJ. Identification of candidate periodontal pathogens and beneficial species by quantitative 16S clonal analysis. J Clin Microbiol. 2005;43:3944–3955. doi: 10.1128/JCM.43.8.3944-3955.2005. - DOI - PMC - PubMed
  10.  
    1. Cortelli JR, Roman-Torres CVG, Aquino DR, Franco GCN, Costa FO, et al. Occurrence of Aggregatibacter actinomycetemcomitans in Brazilians with chronic periodontitis. Braz Oral Res. 2010;24:217–223. doi: 10.1590/S1806-83242010000200015. - DOI - PubMed
  11.  
    1. Wang X, Li L, Yang M, Geng Y, Chen H, et al. Prevalence and distribution of Aggregatibacter actinomycetemcomitans and its cdtB gene in subgingival plaque of Chinese periodontitis patients. BMC Oral Health. 2014;14:14–37. doi: 10.1186/1472-6831-14-37. - DOI - PMC - PubMed
  12.  
    1. Rams TE, Feik D, Listgarten MA, Slots J, Tanner AC. Peptostreptococcus micros in human periodontitis. Oral Microbiol Immunol. 1992;7:1–6. doi: 10.1111/j.1399-302X.1992.tb00011.x. - DOI - PubMed
  13.  
    1. Deng T, Wang L, Lv J, Pang J, Liu B, et al. Association of three bacterial species and periodontal status in Chinese adults: an epidemiological approach. J Clin Microbiol. 2011;49:184–188. doi: 10.1128/JCM.01819-10. - DOI - PMC - PubMed
  14.  
    1. Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol. 2005;43:5721–5732. doi: 10.1128/JCM.43.11.5721-5732.2005. - DOI - PMC - PubMed
  15.  
    1. Dewhirst FE, Chen T, Izard J, Paster BJ, Tanner ACR, et al. The human oral microbiome. J Bacteriol. 2010;192:5002–5017. doi: 10.1128/JB.00542-10. - DOI - PMC - PubMed
  16.  
    1. Dorn BR, Leung KL, Progulske-Fox A, Al-Haroni M, Ghandour I. Invasion of human oral epithelial cells by Prevotella intermedia . Infect Immun. 1998;66:6054–6057. doi: 10.1128/IAI.66.12.6054-6057.1998. - DOI - PMC - PubMed
  17.  
    1. Rotimi VO, Salako NO, Divia M, Asfour L, Kononen E. Prevalence of periodontal bacteria in saliva of Kuwaiti children at different age groups. J Infect Public Health. 2010;3:76–82. doi: 10.1016/j.jiph.2010.02.002. - DOI - PubMed
  18.  
    1. Haffajee AD, Socransky SS, Patel MR, Song X, Kononen E. Microbial complexes in supragingival plaque. Oral Microbiol Immunol. 2008;23:196–205. doi: 10.1111/j.1399-302X.2007.00411.x. - DOI - PubMed
  19.  
    1. Al-Hebshi NN, Al-Sharabi AK, Shuga-Aldin HM, Al-Haroni M, Ghandour I, et al. Effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients. J Ethnopharmacol. 2010;132:564–569. doi: 10.1016/j.jep.2010.08.051. - DOI - PubMed
  20.  
    1. Suzuki N, Yoshida A, Saito T, Kawada M, Nakano Y, et al. Quantitative microbiological study of subgingival plaque by real-time PCR shows correlation between levels of Tannerella forsythensis and Fusobacterium spp. J Clin Microbiol. 2004;42:2255–2257. doi: 10.1128/JCM.42.5.2255-2257.2004. - DOI - PMC - PubMed
  21.  
    1. Martin FE, Nadkarni MA, Jacques NA, Hunter N, Chalabi M. Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. J Clin Microbiol. 2002;40:1698–1704. doi: 10.1128/JCM.40.5.1698-1704.2002. - DOI - PMC - PubMed

Page Navigation